Wortmannin induced apoptosis in iPSCs is caspase dependent.

<p>(A) In support of our FACS analysis, apoptosis was quantified by determination of DNA fragmentation using ELISA cell death detection kit. The both iPSCs BIHi004-A and BIHi001-A were pretreated with QVD-oph or without QVD-oph and subsequently treated for additional 24h with 4μM wortmannin. Insets show picture of the ELISA with respect to color intensity. The color intensity is proportional to the level of apoptotic cells. (B) Pictures were taken 24 h after daily changing medium. (a,c) iPSCs as a control with a lot of dead cells in the supernatant after 24 h in two iPSCs in contrast to iPSCs pretreated with 10 μM caspase inhibitor (b,d).