The effect of Akt2 on DNA-DSB repair is more robust than the effect of Akt1 in H460 cells.

<p>H460 cells that were grown on glass slides were transfected with 50 nM of control-siRNA (siCON), AKT1-siRNA (siAKT1) or AKT2-siRNA (siAKT2). Forty-eight hours (24 h time-point) and 64 h (8 h time-point) after transfection, cells were irradiated with 4 Gy and γ-H2AX assay was performed as described in <i>Materials and Methods</i>. The data represent the mean number of γ-H2AX foci ± SEM from at least 140 cells in two biologically independent experiments. Asterisks indicate statistically significant enhancement of residual γ-H2AX foci following transfection with AKT1- and AKT2-siRNA at the indicated conditions (*, P < 0.05; **, P < 0.01; ***, P < 0.001).