Changes in the amount of free GrybiApoLp-III by AKH stimulation and starvation.

<p>(A) Representative data from native PAGE analyses of proteins in <i>G</i>. <i>bimaculatus</i> hemolymph before and after AKH injection. Free GrybiApoLp-III is indicated by a bar. Figures are representative data from experiments using three individual crickets [the lane number (#1, #2, and #3) indicates the sample from an individual cricket]. Reproducibility of this experiment was confirmed by different experimental trials using more than 30 individuals. (B) Representative data from native PAGE analyses of proteins in hemolymph of starved <i>G</i>. <i>bimaculatus</i>. Free GrybiApoLp-III is indicated by a bar. Numbers represent individual crickets (#1, #2 and #3). Data on the left (samples from fed crickets) is composed of three single gels. The reproducibility of this experiment was also confirmed by different trials using totally more than 30 individual crickets from different populations.