Comparison of whole genome coverage of assembled contigs mapped onto <i>E</i>. <i>coli</i> K12 genome sequences for ddMDA and tube MDA.

<p>(A) From the outermost circle, ddMDA for 100 pg/μL (dark purple), tube MDA for 100 pg/μL (bright purple), 10 pg/μL (dark brown), and 10 pg/μL (bright brown), respectively. GC contents across the genome were depicted in black in the innermost circle and reads from genomic DNA were illustrated in green as a reference. (B) From the outermost circle, ddMDA for 1 pg/μL (dark red), tube MDA for 1 pg/μL (bright red), 0.1 pg/μL (dark blue), and 0.1 pg/μL (bright blue), respectively. (C) GC contents (top) and amplification read depths over the entire E. coli genome for ddMDA (middle) and tube MDA (bottom) at the DNA concentration of 10 pg/μL. The right panels show zoomed-in plots of the dotted-line box region of the genome for close-up visualization.