Dose-dependent inhibition of ZIKV RNA replication by 7DMA.

<p>(<b>A</b>) Vero cell cultures infected with ZIKV strain MR766 were treated with different concentrations of 7DMA. Viral RNA levels in the supernatant were quantified on day 4 pi by means of RT-qPCR and are expressed as percentage inhibition of untreated virus control (black bars). Mock-infected cells were treated with the same dilution series of 7DMA. Cell viability was determined by means of the MTS/PMS method and is expressed as percentage of cell growth of untreated control (white circles). Data represent mean values ± standard deviations (SD) for three independent experiments. Log₁₀ reduction values in viral RNA load are depicted in italics at the top of each bar. (<b>B</b>) Antiviral activity of 7DMA against ZIKV as determined in an immunofluorescence assay. At a concentration of 11 μM, 7DMA almost completely blocked viral antigen expression (left panel) compared to untreated, infected cells (right panel) and infected cells treated at a lower concentration (5.6 and 2.8 μM; two panels in the middle).