Blood vessel density in duodenum and ileum.

<p>(A) Representative image of vWF Immunofluorescence staining in duodenum and ileum of small intestine in control, SMA and PMO25 treated SMA mice. Blood vessels were indicated by vWF (red) staining. DAPI (blue) stains DNA nuclear and was used to outline the intestinal structure. Proportion of vascular density in duodenum (B) and ileum (C). The vascular density was quantified as pixels/unit area using imageJ software. Values in all three groups were then normalized to the mean value in the group of untreated SMA mice. Vascular density was significantly reduced in SMA mice in duodenum (P < 0.001 <i>vs</i> control, P <0.01 <i>vs</i> SMA+PMO25) and ileum (P < 0.05 <i>vs</i> control, P<0.05 <i>vs</i> SMA+PMO25), and was significantly improved after PMO25 treatment. (* P < 0.05, ** P<0.01). Scale bar = 50 μm.