MuLV infection induces selective IDO expression by cells co-expressing DC and B cell markers.

<p><b>AB.</b> Splenocytes from MuLV-infected B6 mice at early (A, 28dpi) or later stages (B, 56dpi) of MuLV infection were stained with anti-CD11c and anti-CD19 mAbs, FACS-sorted to select cell populations and RNA prepared for qPCR analyses to detect IDO1, GAG^Eco or GAG^Def transcripts. <b>C</b>. FACS-sorted CD19⁺ or CD19^neg DCs (closed or open symbols) from MuLV-infected Act-mOVA transgenic mice were cultured with splenocytes from OT1 or OT2 TCR transgenic mice as indicated. After 72hrs, media was analyzed by HPLC to detect Kyn. Statistical significance was evaluated by ANOVA or Student’s <i>t</i> test; ****p<0.0001. <b>DE</b>. Anti-CD4 antibody was used to deplete CD4⁺ T cells (28dpi). Spleen IDO enzyme activity (D) and IDO1 transcription (E) were assessed 5 days later by measuring Kyn release and by qPCR. PCR analyses of sorted splenocytes were performed once per time point, coculture experiments were performed 3 times (C), and CD4 depletion experiments were performed twice.