Production of MCP-1 and proliferation in ARPE-19 cells by treatment with rIL-22.

<p>(A) Expression of IL-22Rα in ARPE-19 cells was analyzed by confocal microscopy. Scale bar = 20 μm (B) ARPE-19 cells were treated with 5 ng/ml and 10 ng/ml of rIL-22 for 48 h. For the last 18 h, 1 μCi of [³H]-thymidine was added to each well. Each sample is in triplicates and results are representative of three independent experiments. (C) ARPE-19 cells were incubated with rIL-22 at 10 ng/ml for 24 h and the concentration of cytokines was measured with ELISA. (D) Migration of PBMCs in response to IL-22 was measured by the migration assay. ARPE-19 cells were placed in the lower chamber with ARPE-19 cells with or without rIL-22 and the upper chamber with PBMCs. PBMCs were then allowed to migrate for 72 h. Anti-MCP-1 Ab at 0.2 μg/ml was used for blocking MCP-1. Data were visualized with Diftquick-staining solution. Scale bar = 200 μm.* p<0.05, ** p<0.001, ***p<0.0001.