Mucosal-Associated Invariant T (MAIT) Cells Are Impaired in Th17 Associated Primary and Secondary Immunodeficiencies - Fig 1

<p>(A) MAIT identification strategy in a healthy control. MAIT cells were identified following sequential gating of CD3 and TCR αβ expression, prior to their Vα7.2 and CD161 co staining. (B) Representative MAIT enumeration from primary and secondary immunodeficiency groups. (C) Mean percentage of MAIT cells (Percentage of MAIT cells in αβ T cells) and conventional T cells (Percentage of conventional T cells in total T cells) present in controls (n = 16, range 1.3–3%), thymoma negative (n = 4, range 0.4–1.9%), thymoma positive (n = 6, range 0.1–0.2%), and HIES (n = 3, range 0.1–1.1%) patients. The percentages of MAIT cells were found to be significantly lower in thymoma positive and HIES patients compared to the healthy controls (*P<0.0001). (D) Representative MR1 expression on control B cell population. Level of MR1 expression on B cell population was not significantly different across the clinical groups.