<p>The fusion proteins were produced in Origami with co-expression of Erv1p/DsbC. Insoluble (Ins), soluble (sol) and IMAC purified fractions are indicated. B) SEC profile of IMAC purified MBP-hST3Gal1-wt and MBP-hST3Gal1-5x.</p
(A) Purified (eluted) rtFhSAP-2 after IMAC (1). (B) Purified (eluted) rtFhFtn-1 after IMAC (2). (C) ...
Purification by IMAC of recombinant enzymes PaAhpC (A), SeAhpC (B), EcTrx (C), EcTrxR (D), HsPrx2 (E...
The maltose binding protein (MBP) fusion protein expression system is a powerful tool to produce and...
<p>A) SEC profile of cleared cell lysate containing overexpressed MBP-hST3Gal1 (black). B) SDS-PAGE ...
<p>Expression of MBP-hST3Gal1 and its purification from each system via IMAC and amylose resin are s...
<p>A) SDS-PAGE of MBP-fused and MBP-cleaved STs. IMAC1: purification of full length fusion proteins;...
<p>A. Overview of the purification process using an MBP affinity column, TEV protease digestion, and...
<p>(A) A flow chart illustrating the purification strategy for the <i>Hm</i>BRI/D94N fusion membrane...
<p>Characterisation of over expressed recombinant fusion protein GST-MC084S and FPLC purified recomb...
Ant (His)-RpoS-DK-G-CSF, which was loaded onto ProBond resin (Ni) column for metal affinity purifica...
<p>(a) 15% Tris/Glycine-SDS-PAGE analysis of the lysates before IPTG induction (t = 0 h) and after I...
<p><b>Copyright information:</b></p><p>Taken from "A family of expression vectors for laboratory sca...
<p><b>Copyright information:</b></p><p>Taken from "A family of expression vectors for laboratory sca...
<p>(A) Culture supernatants of representative yeast clones carrying pPIC9-MBP-fur-GST or pPIC9-MBP-f...
Maltose binding protein (MBP) has a long history as an expression tag for the production of recombin...
(A) Purified (eluted) rtFhSAP-2 after IMAC (1). (B) Purified (eluted) rtFhFtn-1 after IMAC (2). (C) ...
Purification by IMAC of recombinant enzymes PaAhpC (A), SeAhpC (B), EcTrx (C), EcTrxR (D), HsPrx2 (E...
The maltose binding protein (MBP) fusion protein expression system is a powerful tool to produce and...
<p>A) SEC profile of cleared cell lysate containing overexpressed MBP-hST3Gal1 (black). B) SDS-PAGE ...
<p>Expression of MBP-hST3Gal1 and its purification from each system via IMAC and amylose resin are s...
<p>A) SDS-PAGE of MBP-fused and MBP-cleaved STs. IMAC1: purification of full length fusion proteins;...
<p>A. Overview of the purification process using an MBP affinity column, TEV protease digestion, and...
<p>(A) A flow chart illustrating the purification strategy for the <i>Hm</i>BRI/D94N fusion membrane...
<p>Characterisation of over expressed recombinant fusion protein GST-MC084S and FPLC purified recomb...
Ant (His)-RpoS-DK-G-CSF, which was loaded onto ProBond resin (Ni) column for metal affinity purifica...
<p>(a) 15% Tris/Glycine-SDS-PAGE analysis of the lysates before IPTG induction (t = 0 h) and after I...
<p><b>Copyright information:</b></p><p>Taken from "A family of expression vectors for laboratory sca...
<p><b>Copyright information:</b></p><p>Taken from "A family of expression vectors for laboratory sca...
<p>(A) Culture supernatants of representative yeast clones carrying pPIC9-MBP-fur-GST or pPIC9-MBP-f...
Maltose binding protein (MBP) has a long history as an expression tag for the production of recombin...
(A) Purified (eluted) rtFhSAP-2 after IMAC (1). (B) Purified (eluted) rtFhFtn-1 after IMAC (2). (C) ...
Purification by IMAC of recombinant enzymes PaAhpC (A), SeAhpC (B), EcTrx (C), EcTrxR (D), HsPrx2 (E...
The maltose binding protein (MBP) fusion protein expression system is a powerful tool to produce and...
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