Fluorescence activated nuclear sorting (FANS) of three classes of visceral adipose tissue cellular nuclei immuno-stained for PPARg2.

<p><b>A.</b> Histogram of PPARg2-Neg, -Low, -Med. and -High stained nuclei from sorting experiment (log scale) gated for DNA content (DAPI subset, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0154949#pone.0154949.s001" target="_blank">S1B Fig</a>) and forward and side light scattering (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0154949#pone.0154949.s001" target="_blank">S1A Fig</a>). <b>B</b>. <i>PPARg2</i> transcript levels were assayed among the four fractions of nuclei by qRT-PCR. <b>C, D, E, F.</b> Merged immunofluorescence microscope images of four isolated fractions of nuclei without re-staining. <b>G.</b> Comparison of the average nuclear area for the four fractions from one experiment (N = 100). There were statistically significant differences between all of the fractions of nuclear areas except for between PPARg2-Low and PPARg2-Neg fractions. <b>H.</b> PPARg2-High nuclei from image H showing the DAPI staining alone to reveal decondensed nuclei, however, some strongly stained PPARg2-High nuclei are small reflecting some heterogeneity in their morphology, as indicated by white arrows. For antibodies see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0154949#pone.0154949.s006" target="_blank">S1 Table</a>. A p value of P<0.01 is denoted by an asterisk (*) and a p value of P<0.001 is denoted by a double asterisk (**).