Pax6 expression remains unaltered within Sus cells and HBCs upon conditional deletion of <i>Sox2</i>.

<p>Clones from tissue harvested 3-weeks after <i>Cre</i> RVV-transduction of bigenic <i>R26</i>^{<i>fl(stop)-LacZ</i>}<i>; Sox2</i>^{<i>flox/flox</i>} mice were immunolabeled for the neuronal marker PGP9.5 (A) or the HBC marker CD54 (B), along with Pax6 and GFP. In clones containing GFP (+)/ PGP9.5 (-) Sus cells (A), situated above the neural strata (arrows), Pax6 expression remains indistinguishable from surrounding untransduced Sus cells. In clones containing GFP (+)/CD54 (+) HBCs (B), Pax6 levels remain indistinguishable from untransduced HBCs (arrows). Arrowheads mark the basal lamina and scale bar corresponds to 20 μm and applies to all panels.