MOESM12 of Setdb1-mediated H3K9 methylation is enriched on the inactive X and plays a role in its epigenetic silencing

Additional file 12. RNA-seq data confirms correct behaviour of the Xist∆A allele. Reads at Xist are shown for the Xi and Xa from both the MEF (a) and ES (b) cell RNA-seq datasets, showing that the Xist ∆A forces non-random XCI in both MEF and ES cells. (c, d) Box plots showing the transcriptional output, expressed as the total average expression from either the Xi or Xa, of cells transduced with shSetdb1.4 (n = 2), shSetdb1.6 (n = 2) and Nons (n = 2), along a time course of differentiation of Xist ∆A/+ 129/CAST ES cells. Data is shown from replicate 1 (c) and 2 (d). p-values determined by Student’s two-tailed t-test