Surgery impairs vaccine function in a therapeutic B16 melanoma model of minimal residual disease and can be rescued with preoperative IFNα.

<p><b>(a)</b> B6 mice were injected sc with 1x10⁶ B16F10lacZ cells. At day 7, mice were vaccinated with AdDCT. At day 14, tumors were resected (Res) with a 2mm margin +/- surgical stress (Res+Nx). <b>(b)</b> Survival of treated B16F10lacZ tumor-bearing mice are shown in Kaplan-Meier curves. N = 7-8/group. <b>c)</b> Preoperative treatment at day 10 with 1 high dose (10,000 IU/mouse) and at days 11 through 13 with 3 low doses (1000 IU/mouse) of recombinant mIFNα in MRD vaccination model. Survival of treated B16F10lacZ tumor-bearing mice are shown in Kaplan-Meier curves. N = 5-6/group.