Effects of Protein Targeting to Glycogen over-expression on interscapular brown adipose tissue (IBAT) glycogen.

<p>IBAT was excised and snap-frozen from 8-week old mice either <i>ad libitum</i> chow-fed (Fed), 24-hr starved (S), or starved for 24 hr then allowed to refeed for 1, 2, or 4 hr (SR 1, SR 2, SR 4, respectively) before sacrifice. IBAT glycogen (6 mice per condition and time point) (A), and relative gene expressions for genes of interest (5 mice per condition and time point) (B-F) were determined. Error bars are ±SD. Statistics: A Mann-Whitney Rank Sum test was performed between genotypes within each condition (significance indicated above each line indicating the comparison) as well as between the Fed state and each other condition within each genotype (stars directly over the bar). For gene expressions, comparisons were made between Fed and S conditions for each transcript *p<0.05, **p<0.01.