Consensus phosphorylation sites determined by Motif-X analysis of <i>dmc1Δ mek1-as</i> SILAC phosphopeptides.

<p>(A) Motif-X analysis of the 329 Class I peptides with L/H ratios ≥ 2. (B) Motif-X analysis of the 5317 Class 2 peptides with L/H ratios between 0.5–2.0. (C) Motif-X analysis of the 963 Class 3 peptides with L/H ratios < 0.5. Percentages represent the fraction of peptides containing either phosphothreonine or phosphoserine. The size of each amino acid shows how enriched it is relative to the phosphorylated amino acid, and the color indicates physical and chemical properties of the indicated amino acid (Red: acidic; Dark blue: basic; Black: hydrophobic; Yellow: cysteine; Green: proline/Glycine). The percentages of phosphopeptides containing the motifs are indicated. All of the motifs exhibited significant enrichment with a <i>p</i>-value of 0.01.