Palmitoylethanolamide (PEA) failed to significantly affect DSS-induced Akt/mTOR/p70S6K axis activation and HIF-1α expression in PPARα null (KO) mice.

<p><b>(A)</b> Western Blot analysis and <b>(B)</b> relative densitometric analysis showing the effects of PEA (10–100 mg/kg) on the expression of phosphor-Akt, phosphor-mTOR, phosphor-p70S6K and HIF-1α in DSS-treated PPARα null (KO) mice. DSS exposure significantly induced the activation of the pro-angiogenetic Akt/mTOR/p70S6K pathway as compared to untreated mice, but treatment with PEA had no significant effect in treated mice, regardless of the concentration used. Results are expressed as mean ± SD. ***p<0.001 versus untreated mice.