Knockdown of S1PR2 significantly attenuated Nfatc1, Ctsk, Acp5, Oscar, Dcstamp, and Ocstamp mRNA expressions in BM cells with or without treatment with <i>A</i>. <i>actinomycetemcomitans</i>-stimulated media (<i>Aa</i>-media).

<p>BM cells were uninfected, infected with a S1PR2 shRNA lentivirus, or infected with a control shRNA lentivirus (moi 20), and co-cultured with M-CSF and RANKL as described in Methods. A control group of cells were cultured only with M-CSF. BM cells were either unstimulated or stimulated with <i>Aa</i>- media for 4 h. (A) S1PR2 mRNA, (B) Nfatc1 mRNA, (C) Ctsk mRNA, (D) Acp5 mRNA, (E<b>)</b> Oscar mRNA, (F) Dcstamp mRNA, (G) Ocstamp mRNA, (H) RANKL mRNA, (I) RANK mRNA, (J) OPG mRNA, (K) CSF1 mRNA, and (L) CSF1R mRNA levels were quantified by real time PCR and normalized by GAPDH expression. The data are representatives from three separate experiments (n = 3, *<i>P</i><0.05, ***<i>P</i><0.001).