Lam2 physically interacts with Npr2 and Gtr1.

<p>(A) GST-Npr2 and Gtr1-GST were co-precipitated with GFP-Lam2. GFP-Lam2-integrated cells (KP6192) expressing Gtr1-GST (pKB8676), GST-Npr2 (pKB8567) or GST (pKB2437) under the <i>nmt1</i> promoter were grown in EMM medium without thiamine for 20 hr. Gtr1-GST, GST-Npr2 and GST were precipitated from the cell lysates by glutathione beads. The precipitated proteins were subjected to immunoblot analyses using anti-GFP and anti-GST antibodies (Ab). (B) GST-Lam2 and Gtr1-GST were co-precipitated with GFP-Npr2. GFP-Npr2-integrated cells (KP6283) expressing Gtr1-GST (pKB8676), GST-Lam2 (pKB8795) or GST (pKB2437) were grown in EMM medium without thiamine for 20 hr. Gtr1-GST, GST-Lam2 and GST were precipitated from the cell lysates by glutathione beads, and the precipitated proteins were subjected to immunoblot analyses using anti-GFP and anti-GST antibodies (Ab). The asterisks indicate unexpected bands may represent degradation products.