T-cell activation is attenuated by transgelin-2-knockout B-cells during SEB-mediated IS formation.

<p>(A) CD4⁺T-cells and B-cells isolated from <i>TAGLN2</i>^<i>+/+</i> and <i>TAGLN2</i>^<i>-/-</i> mice were combined in four configurations to make T-cell–B-cell conjugates (left table). T-cells were incubated with SEB-loaded or -unloaded B-cells for 24 h, and the levels of mIFN-γ and mIL-2 were measured using ELISA. (B) Each conjugate in (A) was incubated for 12 h and stained with FITC-conjugated anti-mouse CD4 and PE-conjugated anti-mouse CD69 antibodies. Conjugates were pre-gated with CD4 expression, and CD69 expression in T-cells (CD4^high) and B-cells (CD4^low) was assessed by flow cytometry. Graphs depict cumulative data from three independent experiments with each dot representing an individual mouse (n = 9); bars represent means of all data. * <i>p</i> < 0.05 compared with T-cell–B-cell conjugates from TAGLN2^+/+.