LPS binding in indirect ELISA.

<p>FITC-LPSs from <i>E</i>. <i>coli</i> serotypes O111:B4 (1.25 μg/ml) and O55:B5 (2.5 μg/ml), diluted in PBS-EDTA with 0.05% Triton X-100, were added to plates coated with several proteins and peptides (1 μg/well) and blocked with BSA. Data represent the mean values ± SD of duplicate samples. HB, hemoglobin; HF1, histone f1 fraction; LF, lactoferrin; LSZ, lysozyme; MEL, melittin; MF6p, synthetic FhHDM-1/MF6p; MYO, myoglobin; P3L, <i>A</i>. <i>simplex</i> peptide: MCQCVQKYGTEFCKKRLA; PMX, polymyxin B; BSA, bovine serum albumin.