Dicer is solely cytoplasmic during embryogenesis.

<p>(A) Anti-HA immunofluorescence of E13.5 <i>Dcr</i>^<i>FH/FH</i> embryo section. Scale bar is 1 mm. (B-I) Confocal microscopy of immunofluorescence staining of wild type and <i>Dcr</i>^<i>FH/FH</i> E13.5 tissue sections with anti-HA antibody. Sections of lung (B) and liver (C) are shown as representatives of endoderm, vertebrae (D) represent mesoderm, forebrain (E), root ganglion (F), lens (G) and epidermis (H) represent ectoderm, placenta (I) is depicted as an example for an extraembryonic tissue. Nuclei are stained with Hoechst. Scale bar is 10 μm.