Invasion of melanoma cells into tissue engineered skin.

<p>(A) Control TE skin constructs to which fibroblasts and keratinocytes were added to the DED (4x magnification); (B) Skin cells (fibroblasts and keratinocytes) and HBL melanoma cells in DED stained with HM45 antibody staining (40x magnification); (C) Skin cells (fibroblasts and keratinocytes) and A375SM cells stained with S100 antibody staining for A375SM cells (40x magnification); (D) Skin cells (fibroblasts and keratinocytes) and C8161 cells in DED, AE1AE3 antibody staining for keratinocytes only (40x magnification). (DED = De-Epidermised acellular Dermis; F = Fibroblasts; K = Keratinocytes).