PCR amplification of high and low GC oligonucleotides with and without subcycling and varying additives.

<p>A. 10% GC, no sub-cycling. B. 79% GC, no sub-cycling. C. 10% GC, sub-cycling. D. 79% GC, sub-cycling. First lane on the left of each gel: MW markers. Lanes numbered as follows: 1) no additive 2) 40:60 dGTP:deaza-dGTP 3) 50:50 dGTP:deaza-dGTP 4) 60:40 dGTP:deaza-dGTP 5) 2.5% DMSO 6) 10% DMSO 7) 0.1M betaine 8) 0.2M betaine 9) 0.4M betaine 10) 50:50 dGTP:deaza-dGTP with 5% DMSO 11) 60:40 dGTP:deaza-dGTP with 10% DMSO. Results are based on electronic gels created by electrophoresis using a Perkin Elmer GX instrument with a 5k chip. *Bin shows an example of an expected PCR pattern where a strong 200bp product band is seen. PCR reactions were not purified and primers can be seen at the bottom of each sample.