Differential expression and coregulation of biomass degrading genes.

<p>Differential expression (DE) of genes from clades I and III <b>(a)</b> and clade II <b>(b)</b>. Strains were grown with glucose or AFEX pretreated corn stover as the sole carbon source. Each point represents a gene; the shape indicates the strain. The <i>x</i>-axis shows the fold change between carbon sources, and the <i>y</i>-axis shows the statistical support of the fold change. Black points indicate non-significant DE (<i>p</i>-value > 0.05). Red points identify CAZy annotated genes with significant DE. <b>(c)</b> Model for negative transcriptional control of CAZy genes by the CebR transcriptional regulator. The CebR binding sequence is the consensus of the top 25 coexpressed genes from each of the four cellulolytic strains. <b>(d)</b> <i>Streptomyces</i> multilocus phylogenetic tree annotated with quantitative cellulose (filter-paper) degrading activity (red and blue heatmap) and the number of CebR transcriptional regulator binding sites (TGGGAGCGCTCCCA) in the genome (orange bars).