Wavelength-dependent effects on light on sleep induction and sleep duration.

<p>(<b>A</b>) Mice exposed to different wavelengths (405 nm violet, 470 nm blue, or 530 nm green) for 1 hr at ZT14 exhibited differences in sleep onset and sleep duration. (<b>B</b>) Sleep induction is delayed in response to blue light exposure. One-way ANOVA for wavelength, F_(2.23) = 18.791, <i>p</i> ≤ 0.001. Posthoc Tukey violet versus blue <i>p</i> = 0.003, violet versus green <i>p</i> = 0.041, blue versus green <i>p</i> ≤ 0.001. (<b>C</b>) Total sleep duration during the 1 h light pulse is reduced in response to blue light. Data plotted as mean percentage ± SEM (<i>n</i> = 8–10/group). Horizontal black-white-black bar illustrates the light pulse condition from ZT14 until ZT15. One-way ANOVA for wavelength, F_(2.23) = 4.391, <i>p</i> = 0.024. Posthoc Tukey violet versus blue <i>p</i> = 0.046, violet versus green <i>p</i> = 0.517, blue versus green <i>p</i> = 0.008. Statistical differences indicated by *** <i>p</i> ≤ 0.001, ** <i>p</i> ≤ 0.01, * <i>p</i> ≤ 0.05, NS = not significant. The data used to make this figure can be found in <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.1002482#pbio.1002482.s001" target="_blank">S1 Data</a>.