PPIase activities of AquaCyp293 and AquaCyp300.

<p>(A) Kinetics of <i>cis</i>/<i>trans</i> isomerization of 3 μM Abz-Ala-Ala-Pro-Phe-pNa followed by fluorescence at 416 nm, without enzyme (black), with 8 nM (blue), 12 nM (green), 16 nM (dark blue) and 20 nM (red) AquaCyp293. (B) Refolding kinetics of RCM-T1 in the presence of increasing concentrations of AquaCyp293, 0 nM (black), 10 nM (blue), 300 nM (green) and 750 nM (red). The kinetics of refolding of 0.1 μM RCM-T1 in 0.1 M Tris/HCl pH 8.0; 2 M NaCl were measured at 15°C in the presence of various concentrations of AquaCyp293. (C, D) Catalytic efficiencies of AquaCyp293 (○) and AquaCyp300 (□) for (C) the <i>cis</i>/<i>trans</i> isomerization of Abz-Ala-Ala-Pro-Phe-<i>p</i>NA and (D) the refolding of RCM-T1. The measured proline-limited refolding rate constants <i>k</i>_app are shown as a function of the PPIase concentration. The <i>k</i>_cat/<i>K</i>_m values derived from the slopes are given in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0157070#pone.0157070.t001" target="_blank">Table 1</a>.