Functional profiles of <i>C</i>. <i>albicans</i>- and CMV-specific CD4 T-cell responses in healthy donors and HIV-infected subjects.

<p>(A) Functional profile of <i>C</i>. <i>albicans</i>-CD4 T cell responses. Representative flow cytometry data showing expression of each cytokine in CFSE-low, <i>C</i>. <i>albicans</i>-specific CD4 T cells in PBMCs of un-infected healthy (top) or HIV-infected (bottom) subjects. PBMCs of HIV-infected subjects used here were collected during early infection before massive CD4 depletion occurred. Shown are the gated CFSE-low CD4 T cells and the number in each plot shows cytokine+ % in CFSE-low <i>C</i>. <i>albicans</i>-specific CD4 T cells. Comparison for percentage of <i>C</i>. <i>albicans</i>-specific CD4 T cells positive for each cytokine from multiple un-infected and HIV-infected subjects (n = 7) is shown. (B) Functional profile of CMV-specific CD4 T cell responses. Representative flow cytometry data showing expression of each cytokine in CFSE-low, CMV-specific CD4 T cells in PBMCs of un-infected healthy (top) or HIV-infected (bottom) subjects. Comparison for multiple un-infected (n = 7) and infected subjects (n = 6) is also shown. Two-tailed p values are denoted. N.S. represents non-significant.