Cr(VI) induces expression of Nupr1.

<p>(A) Acute chromium exposure induces Nupr1 expression. Total RNA was extracted from BEAS2B cells after a 24 h treatment with 0, 5 or 10 μM chromium. Nupr1 mRNA levels were measured by RT-qPCR. The relative gene expression level was normalized to gamma-tubulin and is presented as fold change compared to the control group. The data shown are the mean ± S.D. from qPCRs performed in triplicate. *, <i>p</i><0.01. (B) Chronic chromium exposure induces Nupr1 expression. After treating BEAS2B cells with 0.5 μM chromium for 1 week or 2 week, the cells were subjected to RT-qPCR using primers for Nupr1. gamma-tubulin was used as an internal control. (C) After Cr(VI) exposure, BEAS2B cells were lysed with RIPA buffer, and whole-cell lysate was run on 14% SDS acrylamide gels. Nupr1 protein levels were then measured with anti-Nupr1 antibody. The band intensities were quantified using ImageJ software.