Expression of TcNTH1 DNA glycosylase in <i>T</i>. <i>cruzi</i> cellular forms.

<p><b>A:</b> TcNTH1 polyclonal antibodies prepared in mice specifically identifies a purified recombinant TcNTH1 (lane 1) and a TcNTH1 expressed in recombinant bacterial homogenates (lane 2). This protein was not recognized in non-expressing bacteria (lane 3). <b>B:</b> Western blot detection of TcNTH1 in total protein homogenates from epimastigotes (lane 1), trypomastigotes (lane 2) and amastigotes (lane 3). Lanes 4 and 5 correspond to TcNTH1 purified from transformed <i>E</i>. <i>coli</i> and to the same protein from recombinant over-expressing <i>T</i>. <i>cruzi</i> epimastigote homogenate, respectively. <b>C:</b> Same as B but using an anti-HIS antibody. <b>D:</b> Loading control for epimastigotes (lane 1), trypomastigotes (lane 2) and amastigotes (lane 3) using an alpha-tubulin antibody. All electrophoretic separations were performed in 12%SDS-PAGE.