Transfection with sRNA52320 reduces LPS-stimulation of IL-8 mRNA abundance and IL-8 cytokine secretion in HBE cells.

<p>(A) HBE cells transfected with sRNA52320 (filled circles) had a lower induction of IL-8 mRNA (ΔCt = control Ct minus LPS-stimulated Ct) in response to LPS than control cells transfected with siNC (open circles). There was a statistically significant difference in the ΔCt means of -0.6 ± 0.2 (95% CI = -1.2 to -0.1, N = 5, p = 0.034 indicated by an asterisk). (B) sRNA52320 reduced IL-8 secretion by HBE cells in response to LPS. The difference in mean IL-8 secretion between cells transfected with siNC (open circles) and sRNA52320 (filled circles) of -149 ± 47 pg/ml was statistically significant (95% CI = -269 to -29, N = 6, p = 0.02 indicated by an asterisk).