Overexpression of <i>OsSAP16</i> Regulates Photosynthesis and the Expression of a Broad Range of Stress Response Genes in Rice (<i>Oryza sativa</i> L.) - Fig 2

<p>A, left border (grey color) of the T-DNA construct was inserted ~1.35kb upstream of LOC_Os07g38240 (OsSAP16) in mutant Ac1 and ~1.79kb in Ac2. The gene (LOC_Os07g38230) upstream of the insertion site encodes an expressed protein with unknown function. B, genotyping of the mutants to identify homozygous (HM) plants in T₄ generation Ac1 mutants (a) and in T₃ generation Ac2 mutants (b). Forward (F) and reverse (R) primers were designed to amplify the genomic sequence either side of the insertion site and the left boarder of the T-DNA construct (BP). The numbers indicate individual progenies for each mutant C, DNA blots show that Ac1 (a) carries a single copy of the construct and Ac2 (b) two. Untransformed rice control plants (DJ) are negative controls for the construct. La is the abbreviation of ladder. D, RT-PCR shows that the expression of LOC_Os07g38240 is higher in T₅ generation Ac1 mutants and in T₄ generation Ac2 mutants compared to T₅ generation DJ, while the expression of LOC_Os07g38230 and the 18s RNA housekeeping gene remains unchanged.