Pathogenic F706 and F779 IgG1 and IgG4 cause loss of cell surface Dsg3.

<p>Primary human keratinocytes were incubated for 8 hours with 50 μg/mL F706/F779 IgG1 or IgG4. Cells were prepared for IF staining using anti-human IgG or anti-Dsg3 (5G11) primary antibodies. Px44 negative and Px43 positive control antibodies were previously characterized [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0156800#pone.0156800.ref022" target="_blank">22</a>]. Scale bar, 20 microns. Results are representative of two independent experiments.