PACAP increases NSC viability in response to hypoglycaemic <i>milieu</i> in correlation with a downward trend in apoptosis.

<p>NSCs were plated as single cells. Prior to 2.5mM glucose addition cells were incubated with PACAP (100 nM) for 10 min. After 24 hours incubation cells were harvested for Western blot experiments <b>(A, B)</b> or intracellular ATP determination <b>(C)</b> or NSC manual counting <b>(D)</b>. To obtain quantitative measurements Bcl-2 protein levels and cleaved caspase 3 were normalized against β-actin. Data are shown as mean ±SEM (A, n = 6–7; B, n = 5–8, C, n = 30–50; D, n = 3–4). Kruskal-Wallis followed by Dunn’s test or Fisher LSD test was used. Differences were considered significant at P<0.05. * denotes P <0.05 compared with control, # denotes P<0.05 compared to 2.5mM glucose.