Pre-depletion of monocytes increases purity of DCs in isolated CD1c⁺ population.

<p>Flow cytometric phenotyping of the resulting CD1c⁺CD14^- and CD1c⁺CD14⁺ populations after application of the CD1c⁺ DC isolation kit without (upper panel) and with (lower panel) pre-depletion of CD14-expressing cells using CD14 MACS micro beads according to the manufacturer’s instructions. Cells are pre-gated on single, live cells. Data are representative for 3 donors.