Human classical and intermediate blood monocytes express CD1c.

<p>(A) Flow cytometric analysis of CD1c expression among Flt3⁺SIRPα⁺DNGR1^- DCs and CD115⁺ monocytes in fresh human PBMCs. Cells are pre-gated on single, live CD45⁺HLA-DR⁺ cells (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0157387#pone.0157387.s001" target="_blank">S1 Fig</a>). Data are representative for 3 donors. (B) Distribution of human blood monocyte subsets characterized by differential expression of CD14 and CD16 and analysis of their CD1c expression levels before (upper panel) and after (lower panel) application of the CD1c⁺ DC isolation kit. Cells are pre-gated on single, live cells (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0157387#pone.0157387.s001" target="_blank">S1 Fig</a>).