Focal remyelination in animals transplanted with EB-NPCs.

<p>(<b>A</b>) Representative brightfield images of coronal spinal cord sections stained with luxol fast blue (LFB) and counter-stained with hemotoxylin and eosin (H&E). (<b>B</b>) Quantification of demyelination in the ventral white matter of EB-NPC, fibroblast, and HBSS injected mice revealed significantly (p < 0.01) reduced demyelination at the injection site in the spinal cords of EB-NPC-transplanted mice. (<b>C</b>) Quantification of demyelination in areas adjacent to the injection site revealed that reduced demyelination was not sustained along the rostrocaudal axis. (<b>D</b>) Representative electron micrographs of coronal spinal cord sections from HBSS, fibroblast, and EB-NPC-injected mice. (<b>E</b>) Analysis of the ratio of the axon diameter vs. total fiber diameter (<i>g</i>-ratio) confirmed enhanced remyelination at the transplant site of EB-NPC-injected mice compared to controls (p < 0.001). For (<b><i>B</i></b>) and (<b><i>C</i></b>), data represents two independent experiments with n = 11 (EB-NPC), n = 8 (Fibroblast), and n = 7 (HBSS) animals per group. For (<b><i>D</i></b>), ≥ 300 axons were measured per experimental group. All data is presented as average ± SEM and was analyzed using one-way ANOVA followed by Tukey’s multiple comparison test.