Anti-EGFR mAb cytotoxicity in combination with NK cells.

<p>The effect of IL-2 and IL-15 activated PBNK cells, cetuximab and panitumumab on lysis of A431 tumor cells was assessed. The percentage of ADCC was calculated based on percentage of PBSE labelled A431 cells staining positive for 7AAD in different co-culture conditions (A). Effector cells from the assay were stained with CD107a to measure NK cell degranulation by flow cytometry (B). Cell free culture supernatants were collected at the end of a 4hr co-culture and analyzed for IFNγ release by ELISA (C). The contribution of FcR mediated effector functions of NK cells (i.e. ADCC) on NK cell mediated A431 tumor cell lysis was tested by blocking the FcR receptor on NK cells (D). Data presented is from six individual PBNK donors. Columns are mean of triplicate values; with bars showing SD. Mean ± SD for each significant condition are represented as p = <0.05 *, <0.01 **, <0.005 ***, <0.001 ****.