Viability of human ACs cultured with expansion, control and chondrogenic media in the self-assembling RAD16-I peptide scaffold and in RAD/CS, RAD/Decorin and RAD/Heparin composites.

<p>(A) Fluorescent images of live/dead staining at week 4 of culture. Live cells are stained in green and dead cells in red (Scale bars = 200 μm). (B) MTT absorbance values of 3D constructs in the three culture media in the four scaffold types at different weeks of culture (Significant differences are indicated as * for p<0.05, ** for p<0.01, and *** for p<0.001, One-way ANOVA, N = 2 n = 3). <b>(C)</b> Construct appearance after MTT incubation at week 4 of culture with the different culture media: expansion (exp), control (con) and chondrogenic (ch) media.