Effect of doxycycline on LDH, cytochrome c oxidase, and total protein release.

<p>A: Confirmation of inhibition of MMP-2 and MMP-9 activity by doxycycline using zymography. B: LDH activity in perfusate of rat kidney with warm ischemic injury at 5, 10, and 22 hours perfused with KPS-1 with and without doxycycline (n = 7 each). C: Cytochrome c oxidase level in perfusate of rat kidney with warm ischemic injury at 5, 10, and 22 hours perfused with KPS-1 with and without doxycycline (n = 7 each). D: Total protein release in perfusate of rat kidney with warm ischemic injury at 5, 10, and 22 hours perfused with KPS-1 with and without doxycycline (n = 6 each). Error bars represent Standard Error of the Mean (SEM). * p<0.05 compared to 5 hours using Student’s T test.# p<0.05 compared to 22 hours using Student’s T test.KPS-1 = Kidney Preservation Solution 1MMP = matrix metalloproteinase.