Leptin receptor (LepR) expression and Tyr (1077) phosphorylation status in liver of HFD-fed mice untreated or treated chronically with rIL-6.

<p>Representative western blot analysis for total LepR and its phosphorylated form (p-LepR) out of five samples per group (STD, HFD and HFD+rIL-6). The corresponding expression of actin is shown as loading control per lane. The ratios for LepR/actin and p-LepR/LepR determined through densitometry are shown in the histograms below. The values represent the means ± SEM. The significance of differences between groups was evaluated using one-way ANOVA. *<i>P</i>< 0.05 and **<i>P</i>< 0.01 denote differences compared with the STD group, ^##<i>P</i>< 0.01, denotes differences compared with HFD group.