Effect of IL-6 treatment on the expression of lipogenic enzymes in the livers of WT mice fed a HFD.

<p><b>(A)</b> The gene expression of <i>Acaca</i>, <i>Acacb</i>, <i>Fasn</i>, and <i>Scd1</i> in the livers of WT fed STD, HFD or HFD-treated chronically with rIL-6 (HFD+rIL-6) is depicted in the corresponding histogram. The gene expression was determined through qPCR analysis of the liver samples. The expression of each gene was normalized using Biogazelle’s qBase^PLUS software with <i>Gapdh</i> and <i>Gus-beta</i> as reference genes. The columns represent CNRQ means ± SEM (n = 8 animals per group). (<b>B</b>) Western blot analysis of the protein expression of Acc-alpha/beta, Fas, and Scd1 in liver samples from WT and mice fed a STD, HFD or HFD+rIL-6. Representative blots of each protein from two samples out of five per group are presented in the upper panel. The corresponding expression of adaptin is shown as a loading control per lane. The histograms below blots depict the levels of each Acc-alpha/beta, Fas and Scd1 protein determined through densitometry and corrected for adaptin. The values represent the means ± SEM (n = 8 samples per group). Comparisons between diet conditions for each gene/protein in A and B were analyzed using one-way ANOVA. *<i>P</i><0.05, **<i>P</i><0.01 and ***<i>P</i><0.001 denote significant differences compared with the corresponding STD-fed group.