The pronounced effect of β3N265M point mutation on etomidate and propofol sensitivity.

<p>(A) Representative traces demonstrating the modulatory effect of 3 μM etomidate and 10 μM propofol on GABA EC₃ (<i>left</i>) and the agonist effect of 30 μM etomidate and 100 μM propofol relative to 10 mM GABA (<i>right</i>) at α1β3γ2L and α1β3N265Mγ2L GABA_ARs. (B) The modulatory and agonist effects of etomidate and propofol were markedly diminished at α1β3N265Mγ2L GABA_ARs. Data are normalised to current responses elicited by 10 mM GABA, and are presented as mean ± SEM. *** <i>p</i> < 0.001; **** <i>p</i> < 0.0001; unpaired <i>t</i> test (mutant <i>vs</i>. wild-type). Numbers above bars indicate number of experiments. GABA + ETO: GABA EC₃ + 3 μM etomidate; ETO: 30 μM etomidate; GABA + PRO: GABA EC₃ + 10 μM propofol; PRO: 100 μM propofol.