Identification of Nedd9 as a TGF-β-Smad2/3 Target Gene Involved in RANKL-Induced Osteoclastogenesis by Comprehensive Analysis - Fig 2

<p>(A) Genes with H3K4me3 peaks within +/- 1 kb from TSS were defined as K4(+) genes, and genes with H3K27me3 peaks within +/- 1 kb from TSS were defined as K27(+) genes. Genes with each combination of K4 and K27 status were identified and enrichment of Smad2/3 target genes was calculated. Highest enrichment was observed in genes with K4(+)K27(+) marks in TGF-ß(-) BMMs and K4(+)K27(-) marks in TGF-ß(+) BMM. (B) The intensity of histone marks around TSS of Smad2/3 target genes. The signal intensity of H3K4me3 in BMMs treated with TGF-ß was higher than those treated with SB431542, while the signal intensity of H3K4me27 was lower in TGF-ß(+) BMMs than that in TGF-ß(-) BMMs. (C) mRNA expression of Smad target genes with K4(+)K27(+) marks in TGF-ß(-) BMMs and K4(+)K27(-) marks in TGF-ß(+) BMM.