Additional file 1: Figure S1. of Targeted optimization of central carbon metabolism for engineering succinate production in Escherichia coli

Assembly of DNA fragment for inserting the T7 RNA polymerase into BW25113 genome. Figure S2. SDS-PAGE of protein expression of strain BKS1, BKS2 and BKS3. Table S1. The simplified central metabolic reaction of engineered E. coli anaerobically grown in glucose. Table S2. Stoichiometric relationships for fluxes of metabolic reactions in anaerobic growth of E. coli. Table S3. Metabolic fluxes (mM gDCW−1 h−1) of engineered strains based on the anaerobic fermentation results at 40 h. Table S4. Primers used in this study. (DOCX 128 kb