Immunohistochemistry of TLR4 and MyD88 in chorionic villi of normal control (n = 36) and GDM (n = 33).

<p>TLR4 protein was expressed in chorionic villi of (A) normal and (B) GDM placentae, a more distinct staining was observed in the trophoblast cell of GDM than the control term placentae (GDM: 0.38±0.01 vs control: 0.27±0.01, p = 0.02). The staining for MyD88 was detected in (C) the normal placentae and (D) the GDM placentae. (E) Negative control. (F) The comparation of TLR4 and MyD88 in placentae between control and GDM. Compared with the control group, enhanced MyD88 staining was shown in GDM (GDM: 0.22±0.02 vs control: 0. 16±0.01, p = 0.03). Sections were counterstained with hematoxylin (blue). The arrows indicate positive cells. All photomicrographs were taken at 400×magnification.