<p>Curves of the chemical denaturation of PmEst with addition of urea (black) and GndHCl (gray), taken from the normalized ellipticity value at 220 nm of the CD spectrum registered at each condition. Sigmoidal fit was performed on each curve, considering two different states for PmEst: native and denatured.</p
<p>SRCD spectra of the enzyme in aqueous solution (black) and in dehydrated films (gray).</p
<p>Unfolding transition curves monitored by (A) ellipticity at 222 nm and (B) the tryptophan fluores...
Denatured states of proteins, the starting points as well as the intermediates of folding in vivo, p...
<p>Urea-induced denaturation of CTD<sub>Mi</sub> mutants. The solid lines represent the fitting curv...
<p>Molar ellipticity at 222 nm ([Θ]<sub>222</sub>) reported after removal of the high-frequency nois...
<p>Denaturation curves were measured for human (open circle), and chimeric (closed circle) Mb. Molec...
<p><b>(A)</b> GdmCl-induced transition between N and D states at 25°C (curve 1) and transition betwe...
<p>Measurements were performed by monitoring the molar ellipticity at ...
<p>a) CTD<sub>Mi</sub>; b) LK-CTD<sub>Mi</sub>; c) RP<sub>Mi</sub>; d) RP-LK-CTD<sub>Mi</sub>. The l...
<p>CD spectra of (A) erythroid spectrin and (B) nonerythroid spectrin showing the native (a) and the...
<p>Urea-induced unfolding curves of far-UV CD spectra of CheW wild-type (black) and R62A mutant (red...
(Panel A) A characteristic Far-UV CD spectra of MsEis was recorded in 20 mM phosphate buffer, pH 8.0...
<p>The [GdmCl]<sub>50%</sub> obtained by fluorescence (red blank circles), by CD (green blank diamon...
<p>(A) The folded protein in the absence of urea (solid line) compared with the unfolded protein in ...
<p>The urea-induced unfolding was analyzed in 20 mM po4 buffer pH7.5, assuming ΔG has a linear depen...
<p>SRCD spectra of the enzyme in aqueous solution (black) and in dehydrated films (gray).</p
<p>Unfolding transition curves monitored by (A) ellipticity at 222 nm and (B) the tryptophan fluores...
Denatured states of proteins, the starting points as well as the intermediates of folding in vivo, p...
<p>Urea-induced denaturation of CTD<sub>Mi</sub> mutants. The solid lines represent the fitting curv...
<p>Molar ellipticity at 222 nm ([Θ]<sub>222</sub>) reported after removal of the high-frequency nois...
<p>Denaturation curves were measured for human (open circle), and chimeric (closed circle) Mb. Molec...
<p><b>(A)</b> GdmCl-induced transition between N and D states at 25°C (curve 1) and transition betwe...
<p>Measurements were performed by monitoring the molar ellipticity at ...
<p>a) CTD<sub>Mi</sub>; b) LK-CTD<sub>Mi</sub>; c) RP<sub>Mi</sub>; d) RP-LK-CTD<sub>Mi</sub>. The l...
<p>CD spectra of (A) erythroid spectrin and (B) nonerythroid spectrin showing the native (a) and the...
<p>Urea-induced unfolding curves of far-UV CD spectra of CheW wild-type (black) and R62A mutant (red...
(Panel A) A characteristic Far-UV CD spectra of MsEis was recorded in 20 mM phosphate buffer, pH 8.0...
<p>The [GdmCl]<sub>50%</sub> obtained by fluorescence (red blank circles), by CD (green blank diamon...
<p>(A) The folded protein in the absence of urea (solid line) compared with the unfolded protein in ...
<p>The urea-induced unfolding was analyzed in 20 mM po4 buffer pH7.5, assuming ΔG has a linear depen...
<p>SRCD spectra of the enzyme in aqueous solution (black) and in dehydrated films (gray).</p
<p>Unfolding transition curves monitored by (A) ellipticity at 222 nm and (B) the tryptophan fluores...
Denatured states of proteins, the starting points as well as the intermediates of folding in vivo, p...