Esterase activity assays.

<p>a) The relative hydrolytic activities of PmEst on substrates with different acyl chain sizes were calculated with respect to C4. b) Determination of the optimum temperature of PmEst on <i>p</i>NB, and c) Residual activity normalized as a function of pre-incubation time at 25, 35, 45 and 55°C. PmEst was inactive when pre-heated at temperatures higher than 55°C. Error bars represent the standard deviations from the triplicate measurements.