ROS production and insulin secretion by pancreatic islets from fed and fasted rats in the presence of 20 mM leucine and 2.8 or 16.7 mM glucose.

<p><b>(A)</b> Mean fluorescence intensity (MFI—arbitrary units) and <b>(B)</b> insulin secretion by pancreatic islets from fed (CT) or 48-hour fasted (48h) rats, treated with dihydroethidium (DHE), and incubated for 60 minutes in the presence of 2.8 mM glucose with or without addition of VAS2870 (20 μM) or DPI (5 μM), associated or not with leucine (LEU—20 mM)–experimental protocol 4. The results are presented as mean ± SEM of four different cell preparations for each group * p <0.05 compared to fed control (CT) under the same conditions as indicated by the Student t-test. <b>(C)</b> Inverse correlation between MFI and insulin secretion in the presence of 2.8 mM glucose with or without addition of VAS2870 (20 μM) or DPI (5 μM) in pancreatic islets isolated from fed or 48-hour fasted rats as indicated by the correlation test using the GraphPad Prism 5.