mPGES-1 expression and PGE₂ production are regulated by H₂S.

<p>Western blot quantifications of microsomal prostaglandin E synthase-1 expression (mPGES-1; normalized to β actin) and representative images are presented. In <b>(A)</b> healthy SV untreated (Ct, black bar) or treated (white bars) with NaHS (10⁻⁵ and 10⁻⁴ mol/L, 24h, n = 4), in (<b>B</b>) healthy aortas (HA, n = 5) and abdominal aneurysmal aortas (AAA, n = 5). PGE₂ content <b>(C)</b> in healthy saphenous veins (SV, n = 14), in small and large diameter varicosities (paired SDv and LDv, n = 14), in <b>(D)</b> healthy aorta (HA, n = 3) and abdominal aortic aneurysm (AAA, n = 3) preparations. PGE₂ concentrations were measured by EIA after 24h incubation with or without the treatments indicated: NaHS (10⁻⁵–10⁻² mol/L, n = 4–5) or CSE inhibitor β-cyano-L-alanine (BCA, 10⁻⁵–10⁻³ mol/L, n = 4–5). *<i>P</i><0.05, ****<i>P</i><0.0001 versus respective untreated healthy vessels without treatment; §<i>P</i><0.05 between SDv and LDv; #<i>P</i><0.05 versus respective control.